Unknown Lab Report

chartless Lab Report: Enterobacter cloacae Jessica Sainvil Professor Cupido Bio 253L1 Thu 9:30-12:30 referable Date: 12/6/12 Unknown#103 Introduction The bacterium Enterobacter cloacae unaffectionate from the reachn #103 was a gee-negative Rod. The purpose of this research lab was to separate and separate the genus and species of an unknown bacterium. It is chief(prenominal) to identify an unknown micro-organism because cognize how the bacterium work and how it is incorporated means knowing how it can affect humans. Unknown bacteria may similarly be employ clinically more pharmaceutical drugs argon based on products made by organisms (Katzung, B.G.2004). In ready to identify the unknown organism a series of tests were per organize. The tests employ were glucose, sucrose, lactose fermentation; Urease, motility, MR-VP, citrate, oxidase, and indole these tests were used to identify the genus of the unknown and a McConkey nutrient agar scale leaf was used to differentiate and confirm the species of the unknown. This draw will explain and give more in astuteness reasons why certain tests were do and the results yielded from them. Materials & Methods On the first daylight after receiving the unknown microorganism a streak habitation was aseptically done as shown in exercise 2 of the cub manual (Kleyn 9).

The streak plate proficiency is done to withdraw a colony formed by a maven cell from a classification containing millions of cells. The streak plate technique was also used to return hold pure cultures of the bacteria victimization a trypticase soy agar (TSA) plate and was i ncubated at 37 ? for 48 hours. Secondly a Gr! am stain was performed as directed from exercise 6in the lab manual (Kleyn 37) and was found to be Gram negative Rods. The Gram stain is important to do because it reveals the morphology of the organism and the formation of the cells; if an organism appears purple below a microscope then it is verbalize to be Gram positive, and if it appears criticise then it is said to be Gram negative. To prevent the organism from getting contaminated,...If you want to get a full essay, order of battle it on our website: BestEssayCheap.com

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